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KMID : 0861020180330010047
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Korea Journal of Herbology
2018 Volume.33 No. 1 p.47 ~ p.55
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Effect of Youngyanggak-san against Thioacetamide Induced Acute Liver Damage in Rat
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Shin Mi-Rae
Kim Kyeong-Jo
Kim Soo-Hyun
Lee Ji-Hye
Kwon Oh-Jun
Roh Seong-Soo
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Abstract
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Objectives : The current study is to evaluate the hepatoprotective effect of youngyanggak-san (YGS) on thioacetamide (TAA)-induced acute liver injury in rats.
Methods : YGS is composed of Glycyrrhizae Radix, Asiasari Radix, Cimicifugae Rhizoma, Saigae Tataricae Cornu. While N-YGS (non-youngyanggak-san) doesn’t include Saigae Tataricae Cornu. Two samples were administrated TAA together for 3 days. Thirty-six rats were divided into four groups. Rats except for the normal group were received TAA (200 ㎎/㎏ of body weight, I.P) were divided into three groups (n=9/group) : Group 1 (TAA only), Group 2 (TAA + 200 ㎎/㎏ YGS) and Group 3 (TAA + 200 ㎎/㎏ N-YGS). Acute liver damage confirmed using histological examination, The factors associated with oxidative stress and liver function activity measured in serum. Also, expressions of inflammation related proteins were investigated by western blot analysis.
Results : Oxidative stress factors such as ROS and ONOO- in the Group 2 was manifested by a significant rise compared with Group 1. YGS markedly decreased the elevated ROS and ONOO-. Furthermore, YGS significantly reduced the levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) The nuclear factor-κB (NF-κB) activation induced by TAA led to increase both inflammatory mediators and cytokines. While YGS administration remarkably suppressed such the overexpression. In addition, the histopathological analysis showed that the liver tissue lesions were improved obviously in YGS treatment.
Conclusion : YGS provided a hepatoprotective effect on acute liver damage through the suppression of oxidative stress. Especially, this effect enhanced markedly when Saigae Tataricae Cornu is included.
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KEYWORD
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Acute liver damage, Youngyanggak-san, Thioacetamide, Oxidative stress, Anti-inflammatory
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